Human Low Affinity Immunoglobulin Gamma Fc Region Receptor 3-A

Mfr.No.

E4857Hu

Sensitivity

2.86ng/ml

Detection Range

5-1000ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A (also known as FCGR3A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD16a;CD16a antigen;Fc-gamma RIII;Fc-gamma RIIIa;Fc-gamma RIII-alpha;FCGR3A;FcR-10;FcRIII;FcRIIIa;IgG Fc receptor III-2;Low affinity immunoglobulin gamma Fc region receptor III-A

Molecular Weight

29089

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4857Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	375.7	10.39	2.8
Intra-Assay	2	18	95.6	4.66	4.9
Intra-Assay	3	18	267.1	13.63	5.1

Background

Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protocol

These standard curves of E4857Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/ml	2.374	2.319	2.285
	2.265
320ng/ml	1.209	1.22	1.186
	1.232
160ng/ml	0.698	0.629	0.595
	0.56
80ng/ml	0.419	0.401	0.367
	0.383
40ng/ml	0.129	0.132	0.098
	0.135
0ng/ml	0.05	0.034	0
	0.018

Gene ID	2214
UniProt ID	P08637

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106131	E4857Hu	48T	$320		Add to Cart
BHE12106131	E4857Hu	96T	$458		Add to Cart

Human Low Affinity Immunoglobulin Gamma Fc Region Receptor 3-A - FCGR3A ELISA Kit

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