Human Long-chain-fatty-acid--CoA Ligase 1

Mfr.No.

E5595Hu

Alternative Names

ACS1;ACSL 1;ACSL1;Acyl-CoA synthetase 1;Arachidonate--CoA ligase;LACS 1;LACS 2;Long-chain acyl-CoA synthetase 1;Long-chain acyl-CoA synthetase 2;Long-chain fatty acid-CoA ligase 2;Long-chain-fatty-acid--CoA ligase 1;Palmitoyl-CoA ligase 1;Palmitoyl-CoA ligase 2;Phytanate--CoA ligase

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

77943

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.26ng/L

Background

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed: 24269233, PubMed: 22633490, PubMed: 21242590). Preferentially uses palmitoleate, oleate and linoleate (PubMed: 24269233). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).

Gene ID	2180
UniProt ID	P33121
Antigen	ACSL1

Research Area

Others

Protocol

These standard curves of E5595Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.109	2.122	2.064
	2.136
1200ng/L	1.765	1.794	1.735
	1.822
600ng/L	1.198	1.241	1.183
	1.285
300ng/L	0.722	0.665	0.607
	0.608
150ng/L	0.438	0.42	0.361
	0.401
0ng/L	0.066	0.058	0
	0.05

Precision

We measured random samples of E5595Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	153.5	6.1	4
Intra-Assay	2	18	1312.4	99.87	7.6
Intra-Assay	3	18	599.2	40.84	6.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106957	E5595Hu	48T	$320		Add to Cart
BHE12106957	E5595Hu	96T	$458		Add to Cart

Human Long-chain-fatty-acid--CoA Ligase 1 - ACSL1 ELISA Kit

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