Human Lipocalin-1 ELISA Kit PicoKine®

Mfr.No.

EK2229

Sensitivity

<60 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	125	250	500	1,000	2,000	4,000	8,000
O.D.	0.088	0.133	0.199	0.307	0.484	0.829	1.254	1.911

Description

Human Lipocalin-1 PicoKine® ELISA Kit (96 Tests). Quantitate Human Lipocalin-1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 60 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H19-D176

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human LCN1

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	239	467	3891	188	427	3569
Standard deviation	9.61	12.24	264.2	14.25	31.38	267.68
CV(%)	4%	2.6%	6.8%	7.6%	7.4%	7.5%

Background

Lipocalin-1 is a protein that in humans is encoded by the LCN1 gene. This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alteatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	239	213	234	221	227	17.28	7.6%
Sample 2	467	451	478	326	431	11.97	2.8%
Sample 3	3891	3425	3196	2685	3299	141.87	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3933
Gene Name	LCN1
UniProt ID	P31025

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001776	EK2229	96 wells/kit	$499		Add to Cart

Human Lipocalin-1 ELISA Kit PicoKine®

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