Human Lim and Calponin Homology Domains-containing Protein 1

Mfr.No.

E5580Hu

Alternative Names

LIM and calponin homology domains-containing protein 1;LIMCH1;LIMCH1A;LMO7B

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

121867

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.13ng/L

Background

Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.

Gene ID	22998
UniProt ID	Q9UPQ0
Antigen	LIMCH1

Research Area

Others

Protocol

These standard curves of E5580Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.382	2.348	2.314
	2.314
600ng/L	1.406	1.354	1.319
	1.302
300ng/L	0.724	0.752	0.718
	0.78
150ng/L	0.303	0.355	0.321
	0.408
75ng/L	0.184	0.154	0.119
	0.123
0ng/L	0.032	0.035	0
	0.037

Precision

We measured random samples of E5580Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	207.87	7.26	3.49
Intra-Assay	2	18	455.08	21.88	4.81
Intra-Assay	3	18	606.32	18.75	3.09

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106942	E5580Hu	48T	$320		Add to Cart
BHE12106942	E5580Hu	96T	$458		Add to Cart

Human Lim and Calponin Homology Domains-containing Protein 1 - LIMCH1 ELISA Kit

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