Human Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4

Mfr.No.

E0240Hu

Description

This sandwich kit is for the accurate quantitative detection of human Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 (also known as LILRB4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD85k;CD85 antigen-like family member K;CD85K;ILT3;ILT-3;Immunoglobulin-like transcript 3;Leukocyte immunoglobulin-like receptor 5;Leukocyte immunoglobulin-like receptor subfamily B member 4;LILRB4;LIR5;LIR-5;Monocyte inhibitory receptor HM18

Reactivity

Human

Detection Range

6-2000ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

49356

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.22ng/L

Background

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Gene ID	11006
UniProt ID	Q8NHJ6
Antigen	LILRB4

Research Area

Others

Protocol

These standard curves of E0240Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.958	2.051	1.996
	2.144
600ng/L	0.965	1.014	0.959
	1.062
300ng/L	0.595	0.606	0.552
	0.618
150ng/L	0.203	0.241	0.187
	0.279
75ng/L	0.116	0.125	0.07
	0.133
0ng/L	0.044	0.054	0
	0.065

Precision

We measured random samples of E0240Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	226.07	9.97	4.41
Intra-Assay	2	18	821.75	22.66	2.76
Intra-Assay	3	18	502.91	23.36	4.64

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101955	E0240Hu	48T	$320		Add to Cart
BHE12101955	E0240Hu	96T	$458		Add to Cart

Human Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 - LILRB4 ELISA Kit

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