Human Leukocyte elastase inhibitor(SERPINB1) ELISA kit

Human Leukocyte elastase inhibitor(SERPINB1) ELISA kit

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Mfr.No.

CSB-EL021065HU

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

serpin peptidase inhibitor, clade B (ovalbumin), member 1, EI, ELANH2, LEI, M/NEI, MNEI, PI2, anti-elastase, protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1

Recovery

The recovery of rat IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-95
EDTA plasma (n=4)	93	89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	100
	Range %	95-104
1:2000	Average %	90
	Range %	86-95
1:4000	Average %	87
	Range %	83-92
1:8000	Average %	101
	Range %	97-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
8000	0.230	0.225	0.228
2000	0.417	0.407	0.412
500	0.643	0.664	0.654
125	1.238	1.257	1.248
31.25	1.754	1.770	1.762
0	2.035	1.988	2.012

Species

Homo sapiens

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P30740

Applications	ELISA
Research Area	SERPINB1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503147	CSB-EL021065HU	96T	$695	Add to Cart
BHE10503147	CSB-EL021065HU	5x96T	$3336	Add to Cart
BHE10503147	CSB-EL021065HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.