Human LBP ELISA Kit PicoKine®

Mfr.No.

EK1271

Sensitivity

<50 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.022	0.053	0.088	0.148	0.342	0.779	1.301	1.985

Description

Human LBP ELISA Kit PicoKine® (96 Tests). Quantitate Human LBP in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Lipopolysaccharide-binding protein;LBP;LBP;

Molecular Weight

53384 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A26-V481

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human LBP

Subcellular Localization

Secreted . Cytoplasmic granule membrane . Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1485	4533	23562	1518	4575	21374
Standard deviation	66.82	271.98	1507.96	85	297.37	1432.05
CV (%)	4.5%	6%	6.4%	5.6%	6.5%	6.7%

Protein Function

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed:7517398, PubMed:24120359). Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed:7517398, PubMed:24120359). .

Background

Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface patte recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1485	1561	1630	1640	1579	62.21	3.9%
Sample 2	4533	5189	4438	4703	4715	289.25	6.1%
Sample 3	23562	22985	21907	22035	22622	683.95	3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3929
Gene Name	LBP
UniProt ID	P18428
Protein Name	Lipopolysaccharide-binding protein
Tissue Specificity	Detected in blood serum (at protein level). .
Sequence	Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Applications	ELISA
Research Area	Defensin,Human Protein,Immunology,Innate Immunity,Macrophage/Inflammation,Microbiology,Protein,TLR Signaling

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001028	EK1271	96 wells/kit	$450		Add to Cart

Human LBP ELISA Kit PicoKine®

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