Human Latent-Transforming Growth Factor Beta-binding Protein 1

Mfr.No.

E4040hu

Description

This sandwich kit is for the accurate quantitative detection of human Latent-transforming Growth Factor Beta-binding Protein 1 (also known as LTBP1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ARCL2E;Latent-transforming growth factor beta-binding protein 1;LTBP1;LTBP-1;TGF-beta1-BP-1;Transforming growth factor beta-1-binding protein 1

Reactivity

Human

Detection Range

10-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

186796

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.41ng/L

Background

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space (PubMed:2022183, PubMed:8617200, PubMed:8939931). Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta (PubMed:8617200, PubMed:8939931, PubMed:15184403). Outcompeted by LRRC32/GARP for binding to LAP regulatory chain of TGF-beta (PubMed:22278742).

Gene ID	4052
UniProt ID	Q14766
Antigen	LTBP1

Research Area

Signal Transduction

Protocol

These standard curves of E4040hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.34	2.288	2.259
	2.236
800ng/L	1.738	1.742	1.714
	1.747
400ng/L	1.051	1.113	1.084
	1.174
200ng/L	0.679	0.681	0.652
	0.682
100ng/L	0.495	0.459	0.431
	0.423
0ng/L	0.028	0.029	0
	0.03

Precision

We measured random samples of E4040hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	245.52	12.27	5
Intra-Assay	2	18	805.35	42.37	5.26
Intra-Assay	3	18	76.66	3.69	4.82

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105460	E4040hu	48T	$320		Add to Cart
BHE12105460	E4040hu	96T	$458		Add to Cart

Human Latent-Transforming Growth Factor Beta-binding Protein 1 - LTBP1 ELISA Kit

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