Human Laminin ELISA Kit PicoKine®

Mfr.No.

EK0434

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.095	0.137	0.168	0.247	0.425	0.624	1.284	2.010

Description

Human Laminin ELISA Kit PicoKine® (96 Tests). Quantitate Human Laminin in cell culture supeatants, cell lysates and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Laminin subunit alpha-1; Laminin A chain; Laminin-1 subunit alpha; Laminin-3 subunit alpha; S-laminin subunit alpha; Laminin subunit beta-1; Laminin B1 chain; Laminin subunit gamma-1; LAMB2

Molecular Weight

337084 MW

Immunogen

Target protein isolated from human fibroblasts

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural human Laminin

Subcellular Localization

Secreted, extracellular space, extracellular matrix, basement membrane. Major component.

Sample Type

Cell culture supeatants, Cell lysates, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	318	1051	4462	295	1015	4289
Standard deviation	23.21	47.29	348.03	26.25	58.87	411.74
CV (%)	7.3%	4.5%	7.8%	8.9%	5.8%	9.6%

Protein Function

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Background

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	318	364	314	373	342	26.47	7.7%
Sample 2	1051	1242	1122	1215	1157	75.9	6.5%
Sample 3	4462	4109	4606	4544	4430	192.37	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	284217, 3908, 3909, 3910, 3911, 3912, 3913, 3914, 22798, 3915, 3918, 10319
Gene Name	LAMA1/LAMB1/LAMC1
UniProt ID	P25391, P24043, Q16787, Q16363, O15230, P07942, P55268, Q13751, A4D0S4, P11047, Q13753, Q9Y6N6
Protein Name	Laminin subunit alpha-1; Laminin subunit beta-1; Laminin subunit gamma-1

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Cell Adhesion Proteins,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Intermediate Filaments,Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000432	EK0434	96 wells/kit	$450		Add to Cart

Human Laminin ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Laminin ELISA Kit PicoKine®

Rate it now..!!