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Home / ELISA Kit

Human L-dopachrome Tautomerase - DCT ELISA Kit

SKU : BHE12102556

Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).

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Bioassay Technology Laboratory

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Mfr.No.

E0881Hu

Sensitivity

0.12ng/ml

Detection Range

0.2-70ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

DCT;DT;L-dopachrome Delta-isomerase;L-dopachrome tautomerase;OCA8;TRP2;TRP-2;Tyrosinase-related protein 2;TYRP2;

Molecular Weight

59145

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0881Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.92	0.23	4.66
Intra-Assay	2	18	15.09	0.77	5.13
Intra-Assay	3	18	24.49	0.96	3.93

Background

Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).

Protocol

These standard curves of E0881Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.374	2.525	2.464
	2.675
20ng/ml	1.554	1.57	1.51
	1.586
10ng/ml	0.903	0.824	0.764
	0.744
5ng/ml	0.377	0.445	0.384
	0.512
2.5ng/ml	0.229	0.219	0.159
	0.209
0ng/ml	0.059	0.06	0
	0.061

Gene ID	1638
UniProt ID	P40126

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102556	E0881Hu	48T	$320		Add to Cart
BHE12102556	E0881Hu	96T	$458		Add to Cart

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