Human Klotho Beta - KLB ELISA Kit

Mfr.No.

E2782Hu

Description

This sandwich kit is for the accurate quantitative detection of human Klotho Beta (also known as KLB) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BetaKlotho;Beta-klotho;BKL;KLB;Klotho beta-like protein

Reactivity

Human

Detection Range

0.5-200nmol/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

119808

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.29nmol/L

Background

Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21 (By similarity).

Gene ID	152831
UniProt ID	Q86Z14
Antigen	KLB

Research Area

Metabolism

Protocol

These standard curves of E2782Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120nmol/L	2.06	2.089	2.021
	2.117
60nmol/L	1.333	1.314	1.247
	1.295
30nmol/L	0.95	0.836	0.769
	0.722
15nmol/L	0.513	0.477	0.41
	0.442
7.5nmol/L	0.234	0.242	0.175
	0.251
0nmol/L	0.068	0.067	0
	0.067

Precision

We measured random samples of E2782Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	83.2	3.48	4.2
Intra-Assay	2	18	18.4	1.09	6
Intra-Assay	3	18	51.6	2.82	5.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104319	E2782Hu	48T	$320		Add to Cart
BHE12104319	E2782Hu	96T	$458		Add to Cart

Human Klotho Beta - KLB ELISA Kit

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