Human KLK5/Kallikrein-5 ELISA Kit PicoKine®

Mfr.No.

EK0817

Sensitivity

<10 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.042	0.165	0.259	0.357	0.803	1.081	1.605	1.988

Description

Human KLK5/Kallikrein 5 ELISA Kit PicoKine® (96 Tests). Quantitate Human KLK5 in cell culture supeatants, serum, plasma (heparin, EDTA) , human milk and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Kallikrein-5;3.4.21.-;Kallikrein-like protein 2;KLK-L2;Stratum coeum tryptic enzyme;KLK5;SCTE;UNQ570/PRO1132;

Molecular Weight

32019 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: V23-S293

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human KLK5

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Human milk, Saliva

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	134	809	2161	146	747	2368
Standard deviation	5.36	33.97	133.98	8.32	32.12	149.18
CV (%)	4%	4.2%	6.2%	5.7%	4.3%	6.3%

Protein Function

May be involved in desquamation.

Background

Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum coeum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	134	128	140	148	137	7.39	5.3%
Sample 2	809	798	776	808	797	13.27	1.6%
Sample 3	2161	2628	2234	2197	2305	188.26	8.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	25818
Gene Name	KLK5
UniProt ID	Q9Y337
Protein Name	Kallikrein-5
Tissue Specificity	Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin. .
Sequence	Belongs to the peptidase S1 family. Kallikrein subfamily.

Applications	ELISA
Research Area	Cancer,Cell Biology,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000634	EK0817	96 wells/kit	$399		Add to Cart

Human KLK5/Kallikrein-5 ELISA Kit PicoKine®

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