Human KLK13/Kallikrein-13 ELISA Kit PicoKine®

Mfr.No.

EK1168

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.003	0.075	0.142	0.229	0.406	0.925	1.209	1.887

Description

Human KLK13/Klk L4 ELISA Kit PicoKine® (96 Tests). Quantitate Human KLK13 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Kallikrein-13;3.4.21.-;Kallikrein-like protein 4;KLK-L4;KLK13;KLKL4;

Molecular Weight

30570 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G17-Q277

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human KLK13

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	212	2051	5005	201	2148	5470
Standard deviation	16.74	159.97	227.92	18.09	186.87	333.67
CV (%)	7.9%	7.8%	4.5%	9%	8.7%	6.1%

Background

Kallikrein-13 is a protein that in humans is encoded by the KLK13 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. By genomic sequence analysis, KLK13 gene is mapped in a 300-kb region on chromosome 19q13.3-q13.4. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components. However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	212	215	228	224	219	6.49	2.9%
Sample 2	2051	2123	1928	1953	2013	78.04	3.8%
Sample 3	5005	5101	4716	5021	4960	145.91	2.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	26085
Gene Name	KLK13
UniProt ID	Q9UKR3
Protein Name	Kallikrein-13
Tissue Specificity	Expressed in prostate, breast, testis and salivary gland.

Applications	ELISA
Research Area	Extracellular Matrix,Invasion/Microenvironment,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000985	EK1168	96 wells/kit	$750		Add to Cart

Human KLK13/Kallikrein-13 ELISA Kit PicoKine®

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