Human KLK11/Kallikrein-11 ELISA Kit PicoKine®

Mfr.No.

EK1166

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.025	0.195	0.301	0.533	0.942	1.351	1.777	2.025

Description

Human KLK11/Kallikrein 11 ELISA Kit PicoKine® (96 Tests). Quantitate Human KLK11 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Kallikrein-11;hK11;3.4.21.-;Hippostasin;Serine protease 20;Trypsin-like protease;Kallikrein-11 inactive chain 1;Kallikrein-11 inactive chain 2;KLK11;PRSS20, TLSP;UNQ649/PRO1279;

Molecular Weight

31059 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-N250

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human KLK11

Subcellular Localization

Isoform 1: Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	217	1054	4888	232	994	4808
Standard deviation	12.8	83.26	240	16.93	92.44	259.63
CV (%)	4.9%	7.9%	4.9%	7.3%	9.3%	5.4%

Protein Function

Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. .

Background

Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alteate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	217	206	233	215	217	9.73	4.4%
Sample 2	1054	1031	1100	1045	1057	25.86	2.4%
Sample 3	4898	4850	4518	5291	4889	274.24	5.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	11012
Gene Name	KLK11
UniProt ID	Q9UBX7
Protein Name	Kallikrein-11
Tissue Specificity	Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). .

Applications	ELISA
Research Area	Cancer,Cell Biology,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000983	EK1166	96 wells/kit	$750		Add to Cart

Human KLK11/Kallikrein-11 ELISA Kit PicoKine®

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