Human Kinesin-like Protein Kif20A

Mfr.No.

E2779Hu

Alternative Names

GG10_2;KIF20A;Kinesin-like protein KIF20A;Mitotic kinesin-like protein 2;MKLP2;Rab6-interacting kinesin-like protein;RAB6KIFL;Rabkinesin-6;RCM6;

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

100278

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.15ng/L

Background

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.

Gene ID	10112
UniProt ID	O95235
Antigen	KIF20A

Research Area

Cell Biology

Protocol

These standard curves of E2779Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.26	2.185	2.132
	2.109
800ng/L	1.931	1.854	1.801
	1.778
400ng/L	1.287	1.239	1.186
	1.192
200ng/L	0.619	0.632	0.578
	0.644
100ng/L	0.375	0.372	0.319
	0.368
0ng/L	0.064	0.053	0
	0.042

Precision

We measured random samples of E2779Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	150.7	6.37	4.23
Intra-Assay	2	18	238.15	10.66	4.47
Intra-Assay	3	18	811.65	50.75	6.25

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104316	E2779Hu	48T	$320		Add to Cart
BHE12104316	E2779Hu	96T	$458		Add to Cart

Human Kinesin-like Protein Kif20A - KIF20A ELISA Kit

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