Human Keratin - Type I Cytoskeletal 16

Mfr.No.

E4226hu

Sensitivity

0.023ng/ml

Detection Range

0.05-9ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Keratin, Type I Cytoskeletal 16 (also known as KRT16) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CK-16;Cytokeratin-16;K16;Keratin, type I cytoskeletal 16;Keratin-16;KRT 16;KRT16

Molecular Weight

51268

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4226hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.1	0.1	3.1
Intra-Assay	2	18	1.4	0.07	5.2
Intra-Assay	3	18	0.8	0.05	5.9

Background

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Protocol

These standard curves of E4226hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4.8ng/ml	2.168	2.115	2.088
	2.062
2.4ng/ml	1.364	1.34	1.313
	1.316
1.2ng/ml	0.663	0.656	0.629
	0.649
0.6ng/ml	0.411	0.397	0.37
	0.383
0.3ng/ml	0.152	0.169	0.142
	0.185
0ng/ml	0.028	0.027	0
	0.025

Gene ID	3868
UniProt ID	P08779

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105625	E4226hu	48T	$320		Add to Cart
BHE12105625	E4226hu	96T	$458		Add to Cart

Human Keratin - Type I Cytoskeletal 16 - KRT16 ELISA Kit

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