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Home / ELISA Kit

Human Keratin - Type 2 Cytoskeletal 8 - KRT8 ELISA Kit

SKU : BHE12106901

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

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Bioassay Technology Laboratory

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Sample Request

Mfr.No.

E5539Hu

Sensitivity

0.025ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CK-8;Cytokeratin-8;K8;Keratin, type II cytoskeletal 8;Keratin-8;KRT 8;KRT8;Type-II keratin Kb8

Molecular Weight

53704

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5539Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4	0.27	6.8
Intra-Assay	2	18	7.5	0.33	4.4
Intra-Assay	3	18	1.2	0.06	5

Background

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Protocol

These standard curves of E5539Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.035	2.185	2.136
	2.334
6ng/ml	1.41	1.393	1.344
	1.376
3ng/ml	0.932	0.903	0.854
	0.874
1.5ng/ml	0.48	0.444	0.395
	0.408
0.75ng/ml	0.285	0.252	0.203
	0.218
0ng/ml	0.033	0.049	0
	0.065

Gene ID	3856
UniProt ID	P05787

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106901	E5539Hu	48T	$320		Add to Cart
BHE12106901	E5539Hu	96T	$458		Add to Cart

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