Human Kallistatin - SERPINA4 ELISA Kit

Mfr.No.

E3392Hu

Description

This sandwich kit is for the accurate quantitative detection of human Kallistatin (also known as SERPINA4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Kallikrein inhibitor;Kallistatin;Peptidase inhibitor 4;PI-4;Serpin A4;SERPINA 4;SERPINA4

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

48542

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.022ng/ml

Background

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.

Gene ID	5267
UniProt ID	P29622
Antigen	SERPINA4

Research Area

Others

Protocol

These standard curves of E3392Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.32	2.357	2.324
	2.393
6ng/ml	1.451	1.323	1.29
	1.194
3ng/ml	0.581	0.578	0.546
	0.575
1.5ng/ml	0.299	0.316	0.284
	0.334
0.75ng/ml	0.161	0.143	0.111
	0.125
0ng/ml	0.044	0.032	0
	0.021

Precision

We measured random samples of E3392Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.6	0.15	2.2
Intra-Assay	2	18	5.5	0.3	5.5
Intra-Assay	3	18	2.9	0.1	3.5

Storage	2-8°C

Reference	Serum Kallistatin and Cholinesterase as Biomarkers for the Diagnosis of Liver Cirrhosis in Patients with Hepatitis C Viral Infection Journal: Clinical Medicine and Diagnostics, 2016; 6(6): 143-152

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104920	E3392Hu	48T	$320		Add to Cart
BHE12104920	E3392Hu	96T	$458		Add to Cart

Human Kallistatin - SERPINA4 ELISA Kit

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