Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®

Mfr.No.

EK1349

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
O.D.	0.011	0.122	0.187	0.314	0.615	0.923	1.435	1.858

Description

Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine® (96 Tests). Quantitate Human F11R in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Junctional adhesion molecule A;JAM-A;Junctional adhesion molecule 1;JAM-1;Platelet F11 receptor;Platelet adhesion molecule 1;PAM-1;CD321;F11R;JAM1, JCAM;UNQ264/PRO301;

Molecular Weight

32583 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S28-V238

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human JAM-A

Subcellular Localization

Cell junction, tight junction . Cell membrane ; Single-pass type I membrane protein . Localized at tight junctions of both epithelial and endothelial cells.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	81	325	1280	73	310	1209
Standard deviation	5.83	16.9	67.84	5.32	16.43	93.09
CV (%)	7.2%	5.2%	5.3%	7.3%	5.3%	7.7%

Protein Function

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. .

Background

Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	81	88	85	93	86	4.38	5%
Sample 2	325	306	319	326	319	7.96	2.4%
Sample 3	1280	1301	1409	1335	1331	48.99	3.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	50848
Gene Name	F11R
UniProt ID	Q9Y624
Protein Name	Junctional adhesion molecule A
Sequence	Belongs to the immunoglobulin superfamily.

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Cell Adhesion,Cytoskeleton/ECM,Platelets,Signal Transduction,Tight Junctions,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000910	EK1349	96 wells/kit	$499		Add to Cart

Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®

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