Human Interleukin-36 Receptor Antagonist Protein

Mfr.No.

E5527Hu

Alternative Names

FIL1;FIL1 delta;FIL1(DELTA);FIL1D;IL-1 delta;IL1F5;IL-1F5;IL1HY1;IL-1HY1;IL1L1;IL-1L1;IL-1ra homolog 1;IL-1-related protein 3;IL-1RP3;IL36RA;IL-36Ra;Interleukin-1 delta;Interleukin-1 family member 5;Interleukin-1 HY1;Interleukin-1 receptor antagonist homolog 1;Interleukin-1-like protein 1;Interleukin-36 receptor antagonist protein;PSORP;PSORS14

Reactivity

Human

Detection Range

3-900ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

16962

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.35ng/L

Background

Inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2 and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus. May activate an anti-inflammatory signaling pathway by recruiting SIGIRR.

Gene ID	26525
UniProt ID	Q9UBH0
Antigen	IL1F5

Research Area

Immunology

Protocol

These standard curves of E5527Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.253	2.172	2.12
	2.091
240ng/L	1.674	1.707	1.655
	1.74
120ng/L	0.998	0.988	0.937
	0.979
60ng/L	0.641	0.68	0.628
	0.719
30ng/L	0.399	0.424	0.373
	0.45
0ng/L	0.056	0.052	0
	0.048

Precision

We measured random samples of E5527Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	121.92	7.99	6.55
Intra-Assay	2	18	251.79	10.58	4.2
Intra-Assay	3	18	46.78	3.09	6.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106889	E5527Hu	48T	$320		Add to Cart
BHE12106889	E5527Hu	96T	$458		Add to Cart

Human Interleukin-36 Receptor Antagonist Protein - IL1F5 ELISA Kit

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