Human Interleukin 25 - IL-25 ELISA Kit

Mfr.No.

E0054Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Interleukin 25 (also known as IL-25) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IL 25;IL-17E;IL25;IL-25;Interleukin-17E;Interleukin-25

Reactivity

Human

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

20330

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.5ng/L

Background

Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

Gene ID	64806
UniProt ID	Q9H293
Antigen	IL25

Research Area

Others

Protocol

These standard curves of E0054Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.072	2.188	2.137
	2.305
1600ng/L	1.274	1.285	1.233
	1.295
800ng/L	0.824	0.781	0.729
	0.737
400ng/L	0.442	0.449	0.398
	0.456
200ng/L	0.22	0.211	0.159
	0.202
0ng/L	0.054	0.051	0
	0.048

Precision

We measured random samples of E0054Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	951.9	59.53	6.3
Intra-Assay	2	18	2350.3	92.6	3.9
Intra-Assay	3	18	693.8	38.7	5.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101789	E0054Hu	96T	$458		Add to Cart
BHE12101789	E0054Hu	48T	$320		Add to Cart

Human Interleukin 25 - IL-25 ELISA Kit

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