Human Interleukin-22 receptor subunit alpha-2(IL22RA2) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL011637HU

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

interleukin 22 receptor, alpha 2, CRF2-10, CRF2-S1, CRF2X, IL-22BP, MGC150509, MGC150510, class II cytokine receptor, interleukin 22-binding protein

Recovery

The recovery of human LGALS9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	83-93
EDTA plasma (n=4)	96	92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-97
1:2	Average %	100
	Range %	95-104
1:4	Average %	88
	Range %	83-94
1:8	Average %	94
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q969J5
Tissue Specificity	Expressed in placenta, spleen, breast, skin and lung. Also detected in intestinal tract, testis, brain, heart and thymus. No expression found in prostate, bladder, kidney, ovary, muscle, bone marrow, liver and uterus. Isoform 1 is expressed only in placen

Applications	ELISA
Research Area	IL22RA2

Storage	Store at 2-8°C. Please refer to protocol.

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