Human Intercellular Adhesion Molecule 3

Mfr.No.

E0266Hu

Description

This sandwich kit is for the accurate quantitative detection of human Intercellular Adhesion Molecule 3 (also known as ICAM-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD50;CDw50;ICAM 3;ICAM3;ICAM-3;ICAM-R;Intercellular adhesion molecule 3

Reactivity

Human

Detection Range

1-400ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

59541

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.41ng/ml

Background

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed: 1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed: 23775590).

Gene ID	3385
UniProt ID	P32942
Antigen	ICAM3

Research Area

Immunology

Protocol

These standard curves of E0266Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.117	2.107	2.052
	2.098
120ng/ml	1.478	1.417	1.361
	1.355
60ng/ml	0.617	0.676	0.62
	0.735
30ng/ml	0.383	0.401	0.345
	0.419
15ng/ml	0.204	0.166	0.11
	0.127
0ng/ml	0.057	0.056	0
	0.055

Precision

We measured random samples of E0266Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	141.6	4.75	3.4
Intra-Assay	2	18	88.3	4.66	5.3
Intra-Assay	3	18	34.8	1.46	4.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101980	E0266Hu	96T	$458		Add to Cart
BHE12101980	E0266Hu	48T	$320		Add to Cart

Human Intercellular Adhesion Molecule 3 - ICAM-3 ELISA Kit

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