Human Integrin Alpha-1 - ITGA1 ELISA Kit

Mfr.No.

E5479Hu

Alternative Names

CD antigen CD49a;CD49 antigen-like family member A;Integrin alpha-1;ITGA 1;ITGA1;Laminin and collagen receptor;VLA-1

Reactivity

Human

Detection Range

0.1-40ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

130848

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.048ng/ml

Background

Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.

Gene ID	3672
UniProt ID	P56199
Antigen	ITGA1

Research Area

Signal Transduction

Protocol

These standard curves of E5479Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.699	1.732	1.687
	1.765
12ng/ml	1.091	1.036	0.992
	0.982
6ng/ml	0.55	0.585	0.54
	0.62
3ng/ml	0.262	0.204	0.159
	0.147
1.5ng/ml	0.15	0.108	0.063
	0.065
0ng/ml	0.058	0.045	0
	0.032

Precision

We measured random samples of E5479Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	22	1.03	4.7
Intra-Assay	2	18	13.8	0.86	6.2
Intra-Assay	3	18	7.7	0.3	3.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106841	E5479Hu	48T	$320		Add to Cart
BHE12106841	E5479Hu	96T	$458		Add to Cart

Human Integrin Alpha-1 - ITGA1 ELISA Kit

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