Human Insulin-like Growth Factor Binding Protein 6

Mfr.No.

E3648Hu

Sensitivity

0.17ng/ml

Detection Range

0.3-90ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Insulin-like Growth Factor Binding Protein 6 (also known as IGFBP-6) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

IBP-6;IGF-binding protein 6;IGFBP 6;IGFBP6;IGFBP-6;Insulin-like growth factor-binding protein 6

Molecular Weight

25322

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E3648Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	26.1	0.61	2.3
Intra-Assay	2	18	18.6	0.89	4.8
Intra-Assay	3	18	10	0.58	5.8

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Activates the MAPK signaling pathway and induces cell migration (PubMed: 24003225).

Protocol

These standard curves of E3648Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.375	2.223	2.177
	2.07
24ng/ml	1.357	1.3	1.253
	1.242
12ng/ml	0.686	0.683	0.637
	0.68
6ng/ml	0.44	0.417	0.371
	0.394
3ng/ml	0.175	0.183	0.137
	0.192
0ng/ml	0.064	0.046	0
	0.029

Gene ID	3489
UniProt ID	P24592

Research Area	Signal Transduction

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12105122	E3648Hu	48T	$320		Add to Cart
BHE12105122	E3648Hu	96T	$458		Add to Cart

Human Insulin-like Growth Factor Binding Protein 6 - IGFBP6 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Insulin-like Growth Factor Binding Protein 6 - IGFBP6 ELISA Kit

Rate it now..!!