Human Immunoglobulin E,IgE ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04980h

Sensitivity

3.640 ng/mL

Detection Range

62.5 ng/mL-4000 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Immunoglobulin E,IgE

Recovery

The recovery of hamster CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-93
EDTA plasma (n=4)	96	87-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of hamster CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	85-96
1:10	Average %	87
	Range %	82-92
1:20	Average %	96
	Range %	89-103
1:40	Average %	98
	Range %	94-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IgE

Storage	Store at 2-8°C. Please refer to protocol.

Reference	A common variant associated with asthma, interleukin 13 R130Q, promotes the production of IgE Chu Y et al,International Journal of Immunogenetics,2012 Aug;39(4):308-313 The correlation of serum chemokine CCL11, CCL17, CCL26, and CCL27 anddisease severity in patients with urticaria/Analysis of serum chemokine levels in patients with HIV-associated eosinophilic folliculitis. Yokobayashi H. et al,J Eur Acad Dermatol Venereol,2013 27(2)

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