Human IL7R/CD127 ELISA Kit PicoKine®

Mfr.No.

EK0713

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.039	0.235	0.412	0.568	1.021	1.617	1.910	2.068

Description

Human IL7R/CD127 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL7R in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-7 receptor subunit alpha;IL-7 receptor subunit alpha;IL-7R subunit alpha;IL-7R-alpha;IL-7RA;CDw127;CD127;IL7R;

Molecular Weight

51581 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-I262

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL7R

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	410	2617	9061	443	2807	9233
Standard deviation	25.42	120.38	516.47	33.22	140.35	627.84
CV (%)	6.2%	4.6%	5.7%	7.5%	5%	6.8%

Protein Function

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

Background

The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	410	395	383	426	403	16.13	4%
Sample 2	2617	2389	2451	2237	2423	136.17	5.6%
Sample 3	9061	9810	8729	10576	9544	712.96	7.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3575
Antigen	IL7R/CD127
Gene Name	IL7R
UniProt ID	P16871
Protein Name	Interleukin-7 receptor subunit alpha
Sequence	Belongs to the type I cytokine receptor family. Type 4 subfamily.

Applications	ELISA
Research Area	Cytokines,Hematopoietic Progenitors,Immunology,Innate Immunity,Interleukins,Lymphoid,Myeloid,Stem Cells,T Lymphocytic Lineage

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000583	EK0713	96 wells/kit	$499		Add to Cart

Human IL7R/CD127 ELISA Kit PicoKine®

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