Human IL1R1/Il 1 Ri ELISA Kit PicoKine®

Mfr.No.

EK0395

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.088	0.123	0.199	0.307	0.484	0.829	1.454	2.011

Description

Human IL1R1/Il 1 Ri ELISA Kit PicoKine® (96 Tests). Quantitate Human IL1R1 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-1 receptor type 1;IL-1R-1;IL-1RT-1;IL-1RT1;CD121 antigen-like family member A;Interleukin-1 receptor alpha;IL-1R-alpha;Interleukin-1 receptor type I;p80;CD121a;Interleukin-1 receptor type 1, membrane form;mIL-1R1;mIL-1RI;Interleukin-1 receptor type 1, soluble form;sIL-1R1;sIL-1RI;IL1R1;IL1R, IL1RA, IL1RT1;

Molecular Weight

65402 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D21-T332

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL1R1

Subcellular Localization

Membrane ; Single-pass type I membrane protein . Cell membrane . Secreted .

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	188	973	4770	205	1005	4361
Standard deviation	8.46	63.24	195.57	10.86	77.38	248.57
CV (%)	4.5%	6.5%	4.1%	5.3%	7.7%	5.7%

Protein Function

Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. .

Background

Interleukin 1 receptor, type I (IL1R1), also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. IL1R1 along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. This protein is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	188	179	191	173	182	7.15	3.9%
Sample 2	973	977	935	964	962	16.42	1.7%
Sample 3	4770	4715	5460	5318	5065	327.7	6.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3554
Gene Name	IL1R1
UniProt ID	P14778
Protein Name	Interleukin-1 receptor type 1
Sequence	Belongs to the interleukin-1 receptor family.

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Cytokines,Hematopoietic Progenitors,Immunology,Innate Immunity,Interleukins,Lymphoid,Myeloid,Stem Cells,T Lymphocytic Lineage,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000404	EK0395	96 wells/kit	$499		Add to Cart

Human IL1R1/Il 1 Ri ELISA Kit PicoKine®

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