Human IL-38/IL1F10 ELISA Kit PicoKine®

Mfr.No.

EK1662

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.072	0.137	0.218	0.386	0.646	1.088	1.638	2.230

Description

Human IL-38/IL1F10 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL1F10 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-1 family member 10; IL-1F10; Family of interleukin 1-theta; FIL1 theta; Interleukin-1 HY2; IL-1HY2; Interleukin-1 theta; IL-1 theta; Interleukin-38; IL-38; IL1F10; FIL1T; IL1HY2; IL38; FKSG75; UNQ6119/PRO20041

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-W152

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-38

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	203	449	1984	197	483	1980
Standard deviation	12.58	26.94	87.29	13.98	38.15	99
CV (%)	6.2%	6%	4.4%	7.1%	7.9%	5%

Protein Function

Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2.

Background

Interleukin-1 family member 10 is a protein that in humans is encoded by the IL1F10 gene. The protein encoded by thi s gene is a member of the interleukin 1 cytokine family. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	203	215	206	177	200	14.13	7%
Sample 2	449	456	455	464	456	5.33	1.1%
Sample 3	1984	2038	1885	1780	1921	98.53	5.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	84639
Antigen	IL-38/IL1F10
Gene Name	IL1F10
UniProt ID	Q8WWZ1
Tissue Specificity	Expressed in fetal skin, spleen and tonsil. Expressed mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil.

Applications	ELISA
Research Area	Cytokines,Immunology,Innate Immunity,Interleukins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001328	EK1662	96 wells/kit	$750		Add to Cart

Human IL-38/IL1F10 ELISA Kit PicoKine®

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