Human IL-23R ELISA Kit PicoKine® (96 Tests). Quantitate Human IL23R in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
There is no detectable cross-reactivity with other relevant proteins.
Specificity
Natural and recombinant human IL-23R
Subcellular Localization
Cell membrane
Sample Type
Cell culture supernatants, Serum, Plasma
Anticoagulant
heparin or EDTA
Precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (pg/ml)
355
1260
6219
384
1339
6060
Standard deviation
19.52
79.38
478.86
28.03
99.08
545.40
CV (%)
5.5%
7.4%
7.7%
7.3%
7.4%
9%
Protein Function
Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.
Background
Interleukin-23 subunit alpha is a protein that in humans is encoded by the IL23A gene. The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.
Reproducibility
Lots
Lot 1 (pg/ml)
Lot 2 (pg/ml)
Lot 3 (pg/ml)
Lot 4 (pg/ml)
Mean (pg/ml)
Standard Deviation
CV (%)
Sample 1
355
334
354
307
337
19.5
5.7%
Sample 2
1260
1269
1408
1249
1296
64.76
4.9%
Sample 3
6219
5300
5785
5269
5643
390.34
6.9%
*number of samples for each test n=16.
Notes
Material Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer . Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Gene ID
149233
Gene Name
IL23R
UniProt ID
Q5VWK5
Tissue Specificity
Expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells.
Applications
ELISA
Research Area
Cell Type Markers,Cytokines,Epigenetics and Nuclear Signaling,Immunoglobulins,Immunology,Innate Immunity,Interleukins,Nuclear Signaling,Nuclear Signaling Pathways,Receptors,Signal Transduction,Signaling Pathway
Storage
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Reviews of Human IL-23R/Interleukin-23 receptor ELISA Kit PicoKine®