Human IL-18R1 ELISA Kit PicoKine®

Mfr.No.

EK1260

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.005	0.096	0.144	0.247	0.593	0.979	1.596	2.047

Description

Human IL-18R1 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL18R1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-18 receptor 1;IL-18R-1;IL-18R1;CD218 antigen-like family member A;CDw218a;IL1 receptor-related protein;IL-1Rrp;IL1R-rp;CD218a;IL18R1;IL1RRP;

Molecular Weight

62304 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: C22-R329

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-18R1

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	73	260	905	69	279	830
Standard deviation	7.25	11.96	57.20	7.37	17.85	60.59
CV (%)	7.2%	4.6%	6.1%	7.5%	6.4%	7.3%

Protein Function

Receptor for interleukin 18 (IL-18). Binding to the agonist leads to the activation of NF-kappa-B.

Background

The interleukin-18 receptor 1 (IL-18R1), also known as CDw218a (cluster of differentiation w218a) or IL18RA, is an interleukin receptor of the immunoglobulin superfamily. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	73	73	69	77	73	2.82	3.8%
Sample 2	260	269	256	285	267	11.14	4.1%
Sample 3	905	862	955	814	884	52.12	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	8809
Gene Name	IL18R1
UniProt ID	Q13478
Protein Name	Interleukin-18 receptor 1
Tissue Specificity	Expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression in Hodgkin disease cell lines.
Sequence	Belongs to the interleukin-1 receptor family.

Applications	ELISA
Research Area	Cytokines,Immunology,Innate Immunity,Interleukins,Macrophage/Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000859	EK1260	96 wells/kit	$499		Add to Cart

Human IL-18R1 ELISA Kit PicoKine®

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