Human IL-17RC ELISA Kit PicoKine®

Mfr.No.

EK0984

Sensitivity

<15 pg/ml

Detection Range

250 pg/ml - 16000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	250	500	1000	2000	4000	8000	16000
O.D.	0.062	0.146	0.232	0.383	0.651	1.083	1.685	2.178

Description

Human IL-17RC ELISA Kit PicoKine® (96 Tests). Quantitate Human IL17RC in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-17 receptor C;IL-17 receptor C;IL-17RC;Interleukin-17 receptor homolog;IL17Rhom;Interleukin-17 receptor-like protein;IL-17RL;ZcytoR14;IL17RC;UNQ6118/PRO20040/PRO38901;

Molecular Weight

86240 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-A454

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-17RC

Subcellular Localization

Cell membrane; Single-pass type I membrane protein. Soluble isoforms may be produced.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	343	2996	7791	360	3053	7459
Standard deviation	24.69	224.7	420.71	32.76	267.51	499.75
CV (%)	7.2%	7.5%	5.4%	9.1%	8.7%	6.7%

Background

Interleukin-17 receptor C is a protein that in humans is encoded by the IL17RC gene. By genomic sequence analysis, IL17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alteatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	343	379	386	353	365	17.78	4.8%
Sample 2	2996	3084	2676	2811	2891	158.82	5.4%
Sample 3	7791	7695	8440	8396	8080	339.55	4.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	84818
Gene Name	IL17RC
UniProt ID	Q8NAC3
Protein Name	Interleukin-17 receptor C
Tissue Specificity	Expressed in brain, cartilage, colon, heart, intestine, kidney, liver, lung, muscle, placenta, and prostate. Low expression in thymus and leukocytes. Expressed (at protein level) in prostate and prostate cancer, skeletal muscle, kidney and placenta. .

Applications	ELISA
Research Area	Cytokines,Immunology,Innate Immunity,Interleukins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000747	EK0984	96 wells/kit	$499		Add to Cart

Human IL-17RC ELISA Kit PicoKine®

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