Human IL-17E/IL-25 ELISA Kit PicoKine®

Mfr.No.

EK0793

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.012	0.070	0.125	0.235	0.420	0.840	1.611	2.011

Description

Human IL-17E/IL-25 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-25;IL-25;Interleukin-17E;IL-17E;IL25;IL17E;UNQ3120/PRO10272;

Molecular Weight

20330 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Y33-G177

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-17E

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	162	689	1026	168	748	1001
Standard deviation	8.91	42.02	50.27	10.92	57.59	53.05
CV (%)	5.5%	6.1%	4.9%	6.5%	7.7%	5.3%

Protein Function

Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

Background

Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-?B activation, and stimulate the production of IL8. Whats more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	162	151	150	157	155	4.84	3.1%
Sample 2	689	601	681	631	650	36.2	5.5%
Sample 3	1026	1036	1126	1127	1078	47.88	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	64806
Antigen	IL-17E/IL-25
Gene Name	IL25
UniProt ID	Q9H293
Protein Name	Interleukin-25
Tissue Specificity	Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
Sequence	Belongs to the IL-17 family.

Applications	ELISA
Research Area	Cytokines,Immunology,Innate Immunity,Interleukins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000917	EK0793	96 wells/kit	$499		Add to Cart

Human IL-17E/IL-25 ELISA Kit PicoKine®

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