Human IL-13/Interleukin-13 ELISA Kit PicoKine®

Mfr.No.

EK0424

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.030	0.057	0.089	0.158	0.294	0.694	1.339	2.022

Description

Human IL-13 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL13 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-13;IL-13;IL13;NC30;

Molecular Weight

15816 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G21-N132

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-13

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	34	190	438	32	176	440
Standard deviation	1.93	10.64	24.09	2.36	10.56	29.92
CV (%)	5.7%	5.6%	5.5%	7.4%	6%	6.8%

Protein Function

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.

Background

Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells which is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4R alpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	34	30	29	33	31	2.06	6.6%
Sample 2	190	180	201	184	188	7.91	4.2%
Sample 3	438	424	460	467	447	17.16	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3596
Gene Name	IL13
UniProt ID	P35225
Protein Name	Interleukin-13
Sequence	Belongs to the IL-4/IL-13 family.

Applications	ELISA
Research Area	Cytokines,Immunology,Innate Immunity,Interleukins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000944	EK0424	96 wells/kit	$499		Add to Cart

Human IL-13/Interleukin-13 ELISA Kit PicoKine®

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