Human IL-12(p70) ELISA Kit PicoKine®

Mfr.No.

EK0421

Sensitivity

<2 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.019	0.069	0.126	0.239	0.441	0.834	1.441	2.086

Description

Human IL-12 (p70) ELISA Kit PicoKine® (96 Tests). Quantitate Human IL-12 (p70) in cell culture supeatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin 12 ;Interleukin 12, P35 ;Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) ;Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35), isoform CRA_a ;IL12A ;hCG_1685939 ;

Molecular Weight

28293 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: (I23-S328) + (R23-S219)

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-12(P70)

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	18	51	294	19	55	310
Standard deviation	1.31	3.41	17.38	1.67	3.79	21.39
CV (%)	7.3%	6.7%	5.2%	8.8%	6.9%	6.9%

Background

Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response.(i) And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.(ii) Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.(iii) The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	18	18	20	17	18	1.08	6%
Sample 2	51	59	60	54	56	3.67	6.5%
Sample 3	294	290	284	296	291	4.58	1.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3592
Gene Name	IL12A
UniProt ID	P29459
Protein Name	Interleukin-12 subunit alpha

Applications	ELISA
Research Area	Cancer,Cytokines,Immunology,Innate Immunity,Interleukins,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000425	EK0421	96 wells/kit	$399		Add to Cart

Human IL-12(p70) ELISA Kit PicoKine®

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