Human IGFBP7/Igfbp Rp1 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0991

Sensitivity

<300 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	625	1,250	2,500	5,000	10,000	20,000	40,000
O.D.	0.029	0.092	0.143	0.237	0.443	0.848	1.570	2.243

Description

Human IGFBP7/Igfbp Rp1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human IGFBP7/Igfbp Rp1 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D30-L282

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human IGFBP7

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1401	2986	17423	1521	3364	17532
Standard deviation	60.24	134.37	731.77	88.22	161.47	806.47
CV(%)	4.3%	4.5%	4.2%	5.8%	4.8%	4.6%

Background

Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors(IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1401	1416	1369	1426	1403	44.90	3.2%
Sample 2	2986	3531	3163	3154	3209	144.38	4.5%
Sample 3	17423	15436	16428	16654	16485	560.5	3.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	3490
Gene Name	IGFBP7
UniProt ID	Q16270

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000290	FEK0991	96 wells/kit	$499		Add to Cart

Human IGFBP7/Igfbp Rp1 PicoKine® Quick ELISA Kit

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Reproducibility

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