Human IGFBP4 PicoKine® ELISA Kit

Mfr.No.

EK0388

Sensitivity

<20 pg/ml

Detection Range

500 pg/ml - 32,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	500	1000	2000	4000	8000	16000	32000
O.D.	0.031	0.169	0.2	0.28	0.401	0.66	1.14	1.880

Description

Human IGFBP4 PicoKine® ELISA Kit (96 Tests). Quantitate Human IGFBP4 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Insulin-like growth factor-binding protein 4;IBP-4;IGF-binding protein 4;IGFBP-4;IGFBP4;IBP4;

Molecular Weight

27934 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D22-E258

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IGFBP4

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	634	3283	15689	630	3596	16628
Standard deviation	29.16	164.15	957.02	40.32	222.95	1330.24
CV (%)	4.6%	5%	6.1%	6.4%	6.2%	8%

Protein Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Background

Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	634	669	599	583	621	33.16	5.3%
Sample 2	3283	3066	3571	3160	3270	190.05	5.8%
Sample 3	15689	15202	16042	14388	15330	620.39	4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3487
Gene Name	IGFBP4
UniProt ID	P22692
Protein Name	Insulin-like growth factor-binding protein 4

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Cytoskeleton/ECM,Extracellular Matrix,Growth Factors,Growth Factors/Hormones,Lipid Signaling,Signal Transduction,Signaling Pathway,Structures

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000972	EK0388	96 wells/kit	$499		Add to Cart

Human IGFBP4 PicoKine® ELISA Kit

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