Human IGFBP-3 ELISA Kit PicoKine®

Mfr.No.

EK0386

Sensitivity

<75 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.034	0.092	0.160	0.230	0.397	0.691	1.251	1.710

Description

Human IGFBP-3 ELISA Kit PicoKine® (96 Tests). Quantitate Human IGFBP3 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

cDNA FLJ31712 fis, clone NT2RI2006445, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA FLJ34387 fis, clone HCHON1000191, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA FLJ38550 fis, clone HCHON2002123, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA PSEC0177 fis, clone OVARC1000287, highly similar to Insulin-like growth factor-binding protein 3 ;

Molecular Weight

31674 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A27-R291

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IGFBP-3

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	271	1607	5480	253	1639	5548
Standard deviation	16.53	69.1	350.72	19.73	95.06	437.8
CV (%)	6.1%	4.3%	6.4%	7.8%	5.8%	7.9%

Background

Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression. Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.

Reproducibility

Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	271	288	290	253	275	14.94	5.4%
Sample 2	1607	1578	1461	1560	1551	54.87	3.5%
Sample 3	5480	5896	5868	5913	5789	179.26	3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3486
Gene Name	IGFBP3
UniProt ID	P17936
Protein Name	Insulin-like growth factor-binding protein 3

Applications	ELISA
Research Area	Angiogenesis,Apoptosis,Associated Proteins,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Cell Death,Diabetes-associated,Growth Factors/Hormones,Metabolism,Obesity,Protein Phosphorylation,Receptors,Signal Transduction,Tyrosine Phosphatases

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000396	EK0386	96 wells/kit	$499		Add to Cart

Human IGFBP-3 ELISA Kit PicoKine®

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