Human IGF-1 ELISA Kit PicoKine®

Mfr.No.

EK0376

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.043	0.081	0.137	0.215	0.406	0.714	1.226	1.817

Description

Human IGF-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human IGF1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Insulin-like growth factor I; IGF-I; Mechano growth factor; MGF; Somatomedin-C; IGF1; IBP1

Molecular Weight

21841 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G49-A118

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with IGF-2.

Specificity

Natural and recombinant human IGF-1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Assay Procedure Summary

Activate the sample

Cell culture supeate: add activating reagent pro rata, i.e. add 20ul of Solution A into 100ul of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6.

Serum, plasma (heparin, EDTA): add activating reagent pro rata, i.e. add 20ul of Solution A into 40ul of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6.

It is unnecessary to activate the recombinant IGF-1.

Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Anticoagulant

heparin or EDTA

Activating Reagent

IGF-1 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant IGF-1.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H₂O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H₂O, add H₂O to adjust volume to 100ml.

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	215	1065	3566	284	1154	4861
Standard deviation	8.82	45.80	164.04	14.48	68.09	325.69
CV (%)	4.1%	4.3%	4.6%	5.1%	5.9%	6.7%

Protein Function

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of (1-14C)-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent teary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.

Background

Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. It is mapped to 12q23.2. The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alteative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	215	211	191	188	201	11.88	5.9%
Sample 2	1065	1091	1134	1073	1090	26.68	2.4%
Sample 3	3566	3691	3369	3307	3483	153.39	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3479
Gene Name	IGF1
UniProt ID	P05019
Protein Name	Insulin-like growth factor I
Sequence	Belongs to the insulin family.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000941	EK0376	96 wells/kit	$499		Add to Cart

Human IGF-1 ELISA Kit PicoKine®

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