Human IFNA2 ELISA Kit PicoKine®

Mfr.No.

EK2127

Sensitivity

<1 pg/ml

Detection Range

3.12 pg/ml - 200 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	3.12	6.25	12.5	25	50	100	200
O.D.	0.049	0.176	0.266	0.417	0.681	1.101	1.657	1.986

Description

Human IFNA2 ELISA Kit PicoKine® (96 Tests). Quantitate Human IFNA2 in cell culture supeatants and serum. Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

IFNA2; IFNA2A; IFNA2B; IFNA2C; Interferon alpha-2; IFN-alpha-2; Interferon alpha-A; LeIF A

Immunogen

Expression system for standard: E.coli; Immunogen sequence: C24-E189

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IFNA2

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	5	19	69	4	21	72
Standard deviation	0.21	0.82	2.69	0.20	1.24	4.10
CV(%)	4.2%	4.3%	3.9%	5.1%	5.9%	5.7%

Background

Interferon alpha-2 is a protein that in humans is encoded by the IFNA2 gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. The encoded protein is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is thought to be a hallmark of severe COVID-19 and may provide a rationale for a combined therapeutic approach.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	5	4	6	5	5	0.11	2.2%
Sample 2	19	22	20	15	19	0.51	2.7%
Sample 3	69	71	75	80	74	2.58	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3440
Gene Name	IFNA2
UniProt ID	P01563
Protein Name	Interferon alpha-2

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001674	EK2127	96 wells/kit	$499		Add to Cart

Human IFNA2 ELISA Kit PicoKine®

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