Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine®

Mfr.No.

EK0373

Sensitivity

<2 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.023	0.073	0.102	0.185	0.357	0.612	1.138	1.901

Description

Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine® (96 Tests). Quantitate Human IFNG in cell culture supeatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interferon gamma;IFN-gamma;Immune interferon;IFNG;

Molecular Weight

19348 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-Q166

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IFN gamma

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	34	150	469	33	139	437
Standard deviation	1.73	8.7	29.54	1.91	9.73	31.9
CV (%)	7.3%	5.8%	7%	5.8%	7%	7.3%

Protein Function

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Background

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	34	38	37	36	36	1.47	4%
Sample 2	150	152	164	166	158	7.07	4.4%
Sample 3	469	446	458	468	460	9.28	2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3458
Gene Name	IFNG
UniProt ID	P01579
Protein Name	Interferon gamma
Tissue Specificity	Released primarily from activated T lymphocytes.
Sequence	Belongs to the type II (or gamma) interferon family.

Applications	ELISA
Research Area	Cancer,Cytokines,Immunology,Innate Immunity,Interferons,Signaling Pathways,Stem Cells,TGF Beta,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000385	EK0373	96 wells/kit	$399		Add to Cart

Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine®

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