Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®

Mfr.No.

EK1452

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.030	0.071	0.165	0.291	0.510	0.802	1.350	1.921

Description

Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® (96 Tests). Quantitate Human IDS in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Iduronate 2-sulfatase;3.1.6.13;Alpha-L-iduronate sulfate sulfatase;Idursulfase;Iduronate 2-sulfatase 42 kDa chain;Iduronate 2-sulfatase 14 kDa chain;IDS;SIDS;

Molecular Weight

61873 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S26-P550

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IDS

Subcellular Localization

Lysosome.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	336	1290	4175	344	1377	4351
Standard deviation	22.84	54.18	324.25	27.86	68.85	417.69
CV (%)	6.8%	4.2%	7.8%	8.1%	5%	9.6%

Protein Function

Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.

Background

Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alteative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	336	299	345	321	325	17.41	5.3%
Sample 2	1290	1156	1326	1367	1284	79.16	6.1%
Sample 3	4175	4541	4703	4646	4516	205.41	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3423
Gene Name	IDS
UniProt ID	P22304
Protein Name	Iduronate 2-sulfatase
Tissue Specificity	Liver, kidney, lung, and placenta.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001054	EK1452	96 wells/kit	$750		Add to Cart

Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®

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