Human ICAM5 ELISA Kit PicoKine®

Mfr.No.

EK1529

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.061	0.130	0.193	0.292	0.586	0.966	1.594	2.053

Description

Human ICAM5 ELISA Kit PicoKine® (96 Tests). Quantitate Human ICAM5 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Intercellular adhesion molecule 5;ICAM-5;Telencephalin;ICAM5;TLCN, TLN;

Molecular Weight

97116 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A28-E570

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ICAM5

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	274	1241	4856	283	1292	5119
Standard deviation	13.97	55.84	233.08	18.39	63.3	250.83
CV (%)	4.9%%	4.5%	4.8%	6.5%	4.9%	4.9%

Protein Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2).

Background

Intercellular adhesion molecule 5 is a protein that in humans is encoded by the ICAM5 gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	274	247	258	248	256	10.84	4.2%
Sample 2	1241	1262	1337	1171	1252	59.17	4.7%
Sample 3	4856	4750	4812	4563	4745	111.75	2.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7087
Gene Name	ICAM5
UniProt ID	Q9UMF0
Protein Name	Intercellular adhesion molecule 5
Tissue Specificity	Expressed on neurons in the most rostral segment of the mammalian brain, the telencephalon.

Applications	ELISA
Research Area	Cell Adhesion Proteins,Cell Type Markers,Membrane Proteins,Neuroscience,Tags & Cell Markers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001112	EK1529	96 wells/kit	$499		Add to Cart

Human ICAM5 ELISA Kit PicoKine®

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