Human ICAM-3 / CD50 ELISA Kit PicoKine®

Mfr.No.

EK0940

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.071	0.124	0.179	0.283	0.498	0.880	1.609	2.399

Description

Human ICAM-3 / CD50 ELISA Kit PicoKine® (96 Tests). Quantitate Human ICAM3 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Intercellular adhesion molecule 3;ICAM-3;CDw50;ICAM-R;CD50;ICAM3;

Molecular Weight

59541 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q30-H485; homodimer

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ICAM-3

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	378	1191	4088	362	1109	4040
Standard deviation	21.92	58.35	290.24	25.7	57.66	335.32
CV (%)	5.8%	4.9%	7.1%	7.1%	5.2%	8.3%

Protein Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.

Background

Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	378	319	323	361	345	25.02	7.2%
Sample 2	1191	1222	1181	1135	1182	31.18	2.6%
Sample 3	4088	4762	4153	4037	4260	292.73	6.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3385
Gene Name	ICAM3
UniProt ID	P32942
Protein Name	Intercellular adhesion molecule 3
Sequence	Belongs to the immunoglobulin superfamily. ICAM family.

Applications	ELISA
Research Area	Adapters,Cell Type Markers,Immunology,Protein Phosphorylation,Signal Transduction,Transmembrane

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000713	EK0940	96 wells/kit	$499		Add to Cart

Human ICAM-3 / CD50 ELISA Kit PicoKine®

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