Human ICAM-3 / CD50 ELISA Kit PicoKine®

Mfr.No.

EK0940

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.071	0.124	0.179	0.283	0.498	0.880	1.609	2.399

Description

Human ICAM-3 / CD50 ELISA Kit PicoKine® (96 Tests). Quantitate Human ICAM3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Intercellular adhesion molecule 3;ICAM-3;CDw50;ICAM-R;CD50;ICAM3;

Molecular Weight

59541 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q30-H485; homodimer

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ICAM-3

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	378	1191	4088	362	1109	4040
Standard deviation	21.92	58.35	290.24	25.7	57.66	335.32
CV (%)	5.8%	4.9%	7.1%	7.1%	5.2%	8.3%

Protein Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.

Background

Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	378	319	323	361	345	25.02	7.2%
Sample 2	1191	1222	1181	1135	1182	31.18	2.6%
Sample 3	4088	4762	4153	4037	4260	292.73	6.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3385
Antigen	ICAM-3
Gene Name	ICAM3
UniProt ID	P32942
Protein Name	Intercellular adhesion molecule 3
Sequence	Belongs to the immunoglobulin superfamily. ICAM family.

Applications	ELISA
Research Area	Adapters,Cell Type Markers,Immunology,Protein Phosphorylation,Signal Transduction,Transmembrane

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000713	EK0940	96 wells/kit	$499		Add to Cart

Human ICAM-3 / CD50 ELISA Kit PicoKine®

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