Human Hydroxyacidoxidase 1

Mfr.No.

E5442Hu

Sensitivity

5.19ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Glycolate oxidase;GOX;HAO 1;HAO1;HAOX1;Hydroxyacid oxidase 1

Molecular Weight

40924

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5442Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	613.5	17.38	2.8
Intra-Assay	2	18	376.5	19.29	5.1
Intra-Assay	3	18	259.6	15.23	5.9

Background

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.

Protocol

These standard curves of E5442Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.386	2.354	2.295
	2.321
600ng/L	1.205	1.26	1.202
	1.316
300ng/L	0.806	0.766	0.708
	0.726
150ng/L	0.474	0.461	0.403
	0.448
75ng/L	0.256	0.281	0.223
	0.306
0ng/L	0.043	0.058	0
	0.074

Gene ID	54363
UniProt ID	Q9UJM8

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106804	E5442Hu	48T	$320		Add to Cart
BHE12106804	E5442Hu	96T	$458		Add to Cart

Human Hydroxyacidoxidase 1 - HAO1 ELISA Kit

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