Human Hyaluronidase-2 - HYAL2 ELISA Kit

Mfr.No.

E2749Hu

Sensitivity

0.014ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

HYAL 2;HYAL2;Hyal-2;Hyaluronidase-2;Hyaluronoglucosaminidase-2;LuCa-2;Lung carcinoma protein 2

Molecular Weight

53860

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2749Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.9	0.28	3.1
Intra-Assay	2	18	1.6	0.1	6.2
Intra-Assay	3	18	5.3	0.32	6

Background

Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R.

Protocol

These standard curves of E2749Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.324	2.245	2.201
	2.167
6ng/ml	1.355	1.327	1.283
	1.299
3ng/ml	0.752	0.776	0.732
	0.801
1.5ng/ml	0.451	0.455	0.411
	0.459
0.75ng/ml	0.312	0.3	0.256
	0.288
0ng/ml	0.048	0.044	0
	0.04

Gene ID	8692
UniProt ID	Q12891

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104286	E2749Hu	48T	$320		Add to Cart
BHE12104286	E2749Hu	96T	$458		Add to Cart

Human Hyaluronidase-2 - HYAL2 ELISA Kit

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