Human Hyaluronan Synthase 1

Mfr.No.

E4172hu

Sensitivity

15.55ng/L

Detection Range

30-9000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Hyaluronan Synthase 1 (also known as HAS1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

HA Synthase 1;HAS 1;HAS1;HuHAS1;Hyaluronan Synthase 1;Hyaluronate Synthase 1;Hyaluronic acid Synthase 1

Molecular Weight

64832

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4172hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1778.7	88.31	5
Intra-Assay	2	18	2791.1	110.42	4
Intra-Assay	3	18	618.4	24.66	4

Background

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate (By similarity).

Protocol

These standard curves of E4172hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.211	2.219	2.18
	2.227
2400ng/L	1.558	1.512	1.473
	1.467
1200ng/L	0.721	0.715	0.676
	0.71
600ng/L	0.446	0.407	0.368
	0.369
300ng/L	0.12	0.157	0.118
	0.194
0ng/L	0.04	0.039	0
	0.038

Gene ID	3036
UniProt ID	Q92839

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105572	E4172hu	48T	$320		Add to Cart
BHE12105572	E4172hu	96T	$458		Add to Cart

Human Hyaluronan Synthase 1 - HAS1 ELISA Kit

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