Human Human 6 phosphofructo 2 kinase/fructose 2 - 6 biphosphatase 3

Mfr.No.

E4884Hu

Alternative Names

6PF-2-K/Fru-2,6-P2ase 3;6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme;6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3;Fructose-2,6-bisphosphatase;IPFK2;iPFK-2;iPFK-2 (Includes: 6-phosphofructo-2-kinase;PFK/FBPase 3;PFK2;PFKFB3;Renal carcinoma antigen NY-REN-56

Reactivity

Human

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

59609

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.25ng/L

Background

PFKFB3 is a gene that encodes the 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 enzyme in humans. It is one of 4 tissue-specific PFKFB isoenzymes identified currently (PFKFB1-4).

Gene ID	5209
UniProt ID	Q16875
Antigen	PFKFB3

Research Area

Cardiovascular

Protocol

These standard curves of E4884Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.194	2.288	2.229
	2.382
1600ng/L	1.134	1.202	1.142
	1.269
800ng/L	0.634	0.702	0.643
	0.771
400ng/L	0.47	0.464	0.404
	0.457
200ng/L	0.241	0.237	0.178
	0.233
0ng/L	0.055	0.059	0
	0.064

Precision

We measured random samples of E4884Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1220.34	79.87	6.54
Intra-Assay	2	18	2151.04	73.93	3.44
Intra-Assay	3	18	789.64	60.07	7.61

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106157	E4884Hu	48T	$320		Add to Cart
BHE12106157	E4884Hu	96T	$458		Add to Cart

Human Human 6 phosphofructo 2 kinase/fructose 2 - 6 biphosphatase 3 - PFKFB3 ELISA Kit

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