HSD3B1 ELISA Kit, 3 beta-hydroxysteroid dehydrogenase ELISA Kit, Delta 5-->4-isomerase type I ELISA Kit, 3-beta-HSD I ELISA Kit, Trophoblast antigen FDO161G ELISA Kit, 3BH ELISA Kit, HSDB3A ELISA Kit
Fine Test
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| Mfr.No. | EH2238 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 0.188ng/ml | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Range | 0.313-20ng/ml | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Reactivity | Human | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Duration | 2 hours | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Type | Competitive | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Description | This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with HSD3B1. HSD3B1 in the sample or standard competes with a fixed amount of biotin-labeled HSD3B1 for sites on a pre-coated antibody specific to HSD3B1. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that tued yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of HSD3B1 in the sample. The concentration of the target substance is inversely proportional to the OD450 value. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Alternative Names | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I ELISA Kit, 3-beta-HSD I ELISA Kit, 3-beta-hydroxy-5-ene steroid dehydrogenase ELISA Kit, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase ELISA Kit, 3-beta-hydroxysteroid 3-dehydrogenase ELISA Kit, Delta-5-3-ketosteroid isomerase ELISA Kit, Dihydrotestosterone oxidoreductase ELISA Kit, Steroid Delta-isomerase ELISA Kit, Trophoblast antigen FDO161G ELISA Kit, HSD3B1 ELISA Kit, 3BH ELISA Kit, HSDB3A ELISA Kit | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Method | Coated with Antigen | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kit Components |
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| Recovery |
Add a certain amount of HSD3B1 into the sample. Calculate the recovery by comparing the measured value with the expected amount of HSD3B1 in the sample.
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| Linearity |
Dilute the sample with a certain amount of HSD3B1 at 1:2, 1:4 and 1:8 to get the recovery range.
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| Specificity | Specifically binds with HSD3B1 , no obvious cross reaction with other analogues. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Standard Curve |
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
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| Sample Type | Tissue lysate, Cell lysates, Cell culture supeatants, Plasma, Other biological fluids | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Components | Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Precision |
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate. Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
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| Background | HSD3B1/3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I(3-beta-HSD I)/Trophoblast antigen FDO161G/3BH/HSDB3A | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Stability |
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
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| Notes | For Research Use Only | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Usage Notes |
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| SKU | Supplier No. | Size | Your price | Quantity | |
|---|---|---|---|---|---|
| BHE10806434 | EH2238 | 48T | $280 |
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| BHE10806434 | EH2238 | 96T | $400 |
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