Human HO-1/HMOX1/HSP32 ELISA Kit PicoKine®

Mfr.No.

EK0888

Sensitivity

<10 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.010	0.056	0.095	0.210	0.407	0.814	1.333	1.863

Description

Human HO-1/HMOX1/HSP32 ELISA Kit PicoKine® (96 Tests). Quantitate Human HMOX1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Heme oxygenase 1;HO-1;1.14.99.3;HMOX1;HO, HO1;

Molecular Weight

32819 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-M288

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human HO-1

Subcellular Localization

Microsome . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	149	658	3760	138	610	3391
Standard deviation	11.62	32.9	180.48	12.69	39.65	200.06
CV (%)	7.8%	5%	4.8%	9.2%	6.5%	5.9%

Protein Function

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Background

HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is localized to chromosome 22. Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	149	156	130	134	142	10.63	7.4%
Sample 2	658	603	678	602	635	33.5	5.2%
Sample 3	3760	3562	3498	3701	3630	104.86	2.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3162
Gene Name	HMOX1
UniProt ID	P09601
Protein Name	Heme oxygenase 1
Tissue Specificity	Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). .

Applications	ELISA
Research Area	Alzheimer's Disease,Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Epigenetics and Nuclear Signaling,Heart Disease,Metabolism,Metabolism Processes,Neurodegenerative Disease,Neurology Process,Neuroscience,NFKB Pathway,Nuclear Signaling,Nuclear Signaling Pathways,Pathways and Processes,Platelets,Response To Hypoxia,Signal Transduction,Signaling Pathway,Vascular Inflammation,Vasculature

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001013	EK0888	96 wells/kit	$499		Add to Cart

Human HO-1/HMOX1/HSP32 ELISA Kit PicoKine®

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